WB
Western blot analysis of JNK phosphorylation in Jurkat whole cell lysate without treatment (A, D), treated with isomycin (B, E), and treated with isomycin and λ protein phosphatase (C, F) The tested antibodies include p-JNK antibodies (14. Thr 183/Tyr 185): (A, B, C) and JNK (D, E, F)| Product Name | Mouse monoclonal antibody to p-JNK (14.Thr 183/Tyr 185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A short amino acid sequence containing Thr 183 and Tyr 185 phosphorylated JNK of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK9,MAPK10 |
|---|---|
| Gene Synonyms | JNK2,JNK3 |
| Gene Summary | JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are sub ject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures. |
| Molecular Weight(MW) | Molecular Weight of p-JNK p46 isoform: 46 kDa. Molecular Weight of p-JNK p54 isoform: 54 kDa. |
WB
Western blot analysis of JNK phosphorylation in Jurkat whole cell lysate without treatment (A, D), treated with isomycin (B, E), and treated with isomycin and λ protein phosphatase (C, F) The tested antibodies include p-JNK antibodies (14. Thr 183/Tyr 185): (A, B, C) and JNK (D, E, F)| Application Notes | WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
Data sheet for OM644359