WB
Western blot analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Intersectin 1/ITSN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
IF analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody. Intersectin 1/ITSN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Intersectin 1/ITSN1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of SiHa cells using anti-Intersectin 1/ITSN1 antibody. Overlay histogram showing SiHa cells stained with anti-Intersectin 1/ITSN1 antibody. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Intersectin 1/ITSN1 Antibody (1:100). DyLight®488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to Intersectin 1/ITSN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Intersectin 1/ITSN1 recombinant protein (Position: A522-P1721). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITSN1 |
|---|---|
| Gene Synonyms | ITSN SH3D1A SH3P17 |
| Gene Full Name | intersectin 1 |
| Gene Summary | The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008] |
| Alternative Names | Intersectin 1 ITSN ITSN1 SH3D1A SH3P17 |
| Molecular Weight(MW) | 195kDa(Observed MW 150kDa) |
| Cellular Localization | Cell membrane,Cell projection,Coated pit,Cytoplasm,Cytoplasmic vesicle,Endosome,Membrane,Nucleus,Synapse,Synaptosome. |
WB
Western blot analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Intersectin 1/ITSN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
IF analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody. Intersectin 1/ITSN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Intersectin 1/ITSN1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of SiHa cells using anti-Intersectin 1/ITSN1 antibody. Overlay histogram showing SiHa cells stained with anti-Intersectin 1/ITSN1 antibody. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Intersectin 1/ITSN1 Antibody (1:100). DyLight®488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
