DB
Genomic extracts from wild-type (WT) or an RNase H-deficient yeast strain (ΔRNH1/ΔRNH201) were spotted on a membrane in a two-fold serial reduction (1,000 to 8 ng per spot from top to bottom) and probed with 1 µg/mL of Anti-DNA-RNA Hybrid. Immunoreactivity was visualized using an anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. Image shows results from duplicate genomic extract preparations from each strain. A significantly enhanced level of DNA-RNA hybrids was observed in extracts from RNase H-deficient yeast strain.ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HeLa ells was performed using a 1:50 dilution of Anti-DNA-RNA Hybrid and visualized with Donkey anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed (Red). Actin filaments have been labeled with Alexa Fluor™ 488-conjugated phalloidin (Green). Nucleus was stained with DAPI (Blue). This antibody immunostained both nuclear and mitochondrial DNA-RNA hybrids.| Product Name | DNA-RNA Hybrid antibody (mAb) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | DBICC/IF |
| DB:1µg/mL ICC/IF:1:50 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Target Description | DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the DNA-RNA Hybrid antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, theDNA-RNA Hybrid antibody is effective in recognizing RNA-DNA hybridization for microarray studies. |
|---|---|
| Alternative Names | #REF! |
DB
Genomic extracts from wild-type (WT) or an RNase H-deficient yeast strain (ΔRNH1/ΔRNH201) were spotted on a membrane in a two-fold serial reduction (1,000 to 8 ng per spot from top to bottom) and probed with 1 µg/mL of Anti-DNA-RNA Hybrid. Immunoreactivity was visualized using an anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. Image shows results from duplicate genomic extract preparations from each strain. A significantly enhanced level of DNA-RNA hybrids was observed in extracts from RNase H-deficient yeast strain.
ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HeLa ells was performed using a 1:50 dilution of Anti-DNA-RNA Hybrid and visualized with Donkey anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed (Red). Actin filaments have been labeled with Alexa Fluor™ 488-conjugated phalloidin (Green). Nucleus was stained with DAPI (Blue). This antibody immunostained both nuclear and mitochondrial DNA-RNA hybrids.| Application Notes | DB:1µg/mL ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
