WB
Western blot analysis of extracts from various samples, using Phospho-PKA alpha/beta/gamma CAT (Thr198) Antibody. Lane 1: Hela cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: Hela cells(heat-shock treatment). Lane 3: Raw264.7 cells(serum starvation treatment).IHC
Phospho-PKA alpha/beta/gamma CAT (Thr198) Antibody at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.IHC
Phospho-PKA alpha/beta/gamma CAT (Thr198) at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-PKA alpha/beta/gamma CAT (Thr198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PRKACA(Accession P17612), corresponding to amino acid residues around phosphorylated Thr198. |
| Modification | P-Thr198 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKACA,PRKACB,PRKACG |
|---|---|
| Gene Synonyms | CAFD1 PKACA PPNAD4 |
| Gene Full Name | protein kinase cAMP-activated catalytic subunit alpha |
| Gene Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] |
| Alternative Names | cAMP dependent protein kinase alpha catalytic subunit cAMP dependent protein kinase beta catalytic subunit cAMP dependent protein kinase catalytic beta subunit isoform 4ab cAMP dependent protein kinase catalytic subunit alpha cAMP dependent protein kinase catalytic subunit alpha isoform 1 cAMP dependent protein kinase catalytic subunit beta cAMP-dependent protein kinase catalytic subunit alpha KAPCA_HUMAN PKA C alpha PKA C beta PKA C-alpha PKACA PKACB PPNAD4 PRKACA PRKACAA PRKACB Protein kinase A catalytic subunit alpha Protein kinase A catalytic subunit Protein kinase A catalytic subunit beta Protein kinase cAMP dependent catalytic alpha Protein kinase cAMP dependent catalytic beta cAMP-dependent protein kinase catalytic beta subunit isoform 4ab cAMP-dependent protein kinase catalytic subunit beta KAPCB_HUMAN PKA C beta PKA C-beta PKACB Prkacb protein kinase A catalytic subunit beta Protein kinase cAMP dependent catalytic beta cAMP-dependent protein kinase catalytic subunit gamma KAPCG_HUMAN KAPG PKA C gamma PKA C-gamma PKACg PRKACG Protein kinase cAMP dependent catalytic gamma Serine (threonine) protein kinase |
| Molecular Weight(MW) | 40kDa |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Membrane>Lipid-anchor. |
WB
Western blot analysis of extracts from various samples, using Phospho-PKA alpha/beta/gamma CAT (Thr198) Antibody. Lane 1: Hela cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: Hela cells(heat-shock treatment). Lane 3: Raw264.7 cells(serum starvation treatment).
IHC
Phospho-PKA alpha/beta/gamma CAT (Thr198) Antibody at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
IHC
Phospho-PKA alpha/beta/gamma CAT (Thr198) at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
