WB
Sample loading: 2 ug total protein of Arabidopsis/rice leaves, Electrophoresis: 12.5% SDS-PAGE, Membrane transfer: NC (nitrocellulose) membrane, voltage 10 V overnight, Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour, Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour.| Product Name | Anti-RBCL / Ribulose bisphosphate carboxylase large subunit |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana ATCG00490. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | rbcL |
|---|---|
| Gene Full Name | ribulose bisphosphate carboxylase large subunit |
| Gene Summary | Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. The enzyme usually consists of two types of protein subunit, called the large chain (RBCL) and the small chain (RBCS). |
| Molecular Weight(MW) | 53 / 53-55 kDa for Arabidopsis thaliana, 53 / 53 kDa for rice. |
WB
Sample loading: 2 ug total protein of Arabidopsis/rice leaves, Electrophoresis: 12.5% SDS-PAGE, Membrane transfer: NC (nitrocellulose) membrane, voltage 10 V overnight, Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour, Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour.| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
