WB
Sample: 1 Transforming BIP-GFP plants into tobacco transient expression; 2. Tobacco leaf electrophoresis before transformation: 10% SDS-PAGE membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight blocking: 5% skim milk powder, room temperature incubation for 1 hour primary antibody: 1:5000 dilution, room temperature incubation for 1 hour