WB
All lanes : Anti-HPV11 E7 antibody at 1:1000 dilution, Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein, Lane 2 : lysate of plain U20S cells, Lane 3 : lysate of HeLa cells, Lane 5 : marker, Predicted band size: 11 kDa.ICC/IF
Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells. IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7. IF analysis of E7 protein shows similar result also in HeLa cells.| Product Name | Mouse Monoclonal antibody to HPV11 E7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant full length HPV11 E7 protein purified from bacteria. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000 ICC/IF:Use at an assay dependent dilution. ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human papillomavirus |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Alternative Names | Human papillomavirus E7 protein antibody |
|---|---|
| Molecular Weight(MW) | 11kDa |
| Function | Infections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation. |
WB
All lanes : Anti-HPV11 E7 antibody at 1:1000 dilution, Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein, Lane 2 : lysate of plain U20S cells, Lane 3 : lysate of HeLa cells, Lane 5 : marker, Predicted band size: 11 kDa.
ICC/IF
Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells. IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7. IF analysis of E7 protein shows similar result also in HeLa cells.| Application Notes | WB:1:1000 ICC/IF:Use at an assay dependent dilution. ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
