WB
Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody at 1/1,000 dilution. Lane 1: HEK-293-NT cell lysate, Lane 2: HEK-293-OE-SCXA cell lysate, Lane 3: HeLa-NT cell lysate, Lane 4: HeLa-OE-SCXA cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa transfected with or without SCXA cells labeling SCXA with Rabbit anti-SCXA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCXA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | SCXA Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SCXA aa 1-201 / 201. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCX |
|---|---|
| Gene Synonyms | SCXA SCXB bHLHa48 |
| Gene Full Name | scleraxis bHLH transcription factor |
| Gene Summary | Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex. [provided by Alliance of Genome Resources, Jun 2025] |
| Alternative Names | Basic helix loop helix transcription factor scleraxis antibody Basic helix-loop-helix transcription factor scleraxis antibody bHLHa41 antibody bHLHa48 antibody Class A basic helix-loop-helix protein 41 antibody Class A basic helix-loop-helix protein 48 antibody scleraxis antibody scleraxis homolog A antibody SCX antibody SCX_HUMAN antibody Basic helix loop helix transcription factor scleraxis antibody Basic helix-loop-helix transcription factor scleraxis antibody bHLHa41 antibody bHLHa48 antibody Class A basic helix-loop-helix protein 41 antibody Class A basic helix-loop-helix protein 48 antibody scleraxis antibody scleraxis homolog A antibody SCX antibody SCX_HUMAN antibody SCXB antibody |
| Molecular Weight(MW) | 22kDa(Observed band size: 27kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody at 1/1,000 dilution. Lane 1: HEK-293-NT cell lysate, Lane 2: HEK-293-OE-SCXA cell lysate, Lane 3: HeLa-NT cell lysate, Lane 4: HeLa-OE-SCXA cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa transfected with or without SCXA cells labeling SCXA with Rabbit anti-SCXA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCXA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
