IHC
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining.| Product Name | SCXA Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human SCXA : 131-201/201 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:500 |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCX |
|---|---|
| Gene Synonyms | SCXA SCXB bHLHa48 |
| Gene Full Name | scleraxis bHLH transcription factor |
| Gene Summary | Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SCX bHLHa41 SCXA SCXB bHLHa48 Scl SCX_HUMAN Class A basic helix-loop-helix protein 41 (bHLHa41) Class A basic helix-loop-helix protein 48 (bHLHa48) SCX_MOUSE scleraxis bHLH transcription factor scleraxis homolog A (mouse) scleraxis homolog B (mouse) scleraxis homolog (mouse) scleraxis basic helix-loop-helix transcription factor |
| Molecular Weight(MW) | 22 kDa |
IHC
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining.| Application Notes | IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
