WB
Western blot analysis of biotinylated goat IgG (A) and normal goat IgG (B)ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin revealed mitochondrial localization of biotinylated proteins.ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin revealed mitochondrial localization of biotinylated proteins| Product Name | Mouse Monoclonal antibody to Biotin |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | All |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Target Description | Biotin, a water-soluble B complex vitamin, is required by all organisms but can only be synthesized by yeasts, molds, algaes, some plant species and bacteria. Biotin, a tetrahydrothiophene ring fused with an ureido (tetrahydro imidizalone) ring, is important in the catalysis of essential metabolic reactions to synthesize fatty acids, to metabolize leucine and in gluconeogenesis. Human intestinal bacteria generally produce in excess of the body’s daily Biotin requirement. The occurrence of Biotin in nature is widespread and, although extremely rare, Biotin deficiency is associated with dermatitis, nausea, loss of hair, depression, muscle pain and reproductive disturbances. |
|---|---|
| Alternative Names | Vitamin B4 |
WB
Western blot analysis of biotinylated goat IgG (A) and normal goat IgG (B)
ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin revealed mitochondrial localization of biotinylated proteins.
ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin revealed mitochondrial localization of biotinylated proteins| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
