WB
Western Blot analysis of 1 Jurkat treated with LPS, 2 Jurkat ,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.IHC
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).| Product Name | PI3 Kinase Class III (Phospho Ser249) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized phosho peptide around human PI3 Kinase Class III (Ser249) |
| Modification | p-Ser249 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:50-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3C3 |
|---|---|
| Gene Synonyms | VPS34 Vps34 hVps34 |
| Gene Full Name | phosphatidylinositol 3-kinase catalytic subunit type 3 |
| Gene Summary | Enables 1-phosphatidylinositol-3-kinase activity. Involved in several processes, including early endosome to late endosome transport; macroautophagy; and phosphatidylinositol-3-phosphate biosynthetic process. Acts upstream of or within autophagy and positive regulation of protein lipidation. Located in autolysosome; late endosome; and midbody. Part of phosphatidylinositol 3-kinase complex, class III. [provided by Alliance of Genome Resources, Jun 2025] |
| Alternative Names | Phosphatidylinositol 3-kinase catalytic subunit type 3 PI3-kinase type 3 PI3K type 3 PtdIns-3-kinase type 3 Phosphatidylinositol 3-kinase p100 subunit Phosphoinositide-3-kinase class 3 hVps34 |
| Molecular Weight(MW) | 100kDa |
| Cellular Localization | Midbody,Late endosome, Cytoplasmic vesicle, autophagosome . |
WB
Western Blot analysis of 1 Jurkat treated with LPS, 2 Jurkat ,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.
IHC
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).| Application Notes | WB:1:500-1:2000 IHC:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
