WB
All lanes: PYGL antibody at 2µg/ml, Lane 1: 293T whole cell lysate, Lane 2: HepG2 whole cell lysate, Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution.IHC
Immunohistochemistry of paraffin-embedded human liver tissue using PYGL antibody at dilution of 1:100.IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using PYGL antibody at dilution of 1:100.| Product Name | Rabbit anti-PYGL Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Glycogen phosphorylase, liver form protein (2-846 AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PYGL |
|---|---|
| Gene Synonyms | GSD6 |
| Gene Full Name | glycogen phosphorylase L |
| Gene Summary | This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011] |
| Alternative Names | Glycogen phosphorylase antibody Glycogen phosphorylase L antibody Glycogen phosphorylase liver antibody Glycogen phosphorylase liver form antibody GSD6 antibody Hers disease glycogen storage disease type VI antibody liver form antibody OTTHUMP00000233649 antibody OTTHUMP00000233651 antibody Phosphorylase glycogen liver antibody Pygl antibody PYGL_HUMAN antibody |
| Molecular Weight(MW) | 98kDa |
| Cellular Localization | Cytoplasm, cytosol. |
WB
All lanes: PYGL antibody at 2µg/ml, Lane 1: 293T whole cell lysate, Lane 2: HepG2 whole cell lysate, Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human liver tissue using PYGL antibody at dilution of 1:100.
IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using PYGL antibody at dilution of 1:100.| Application Notes | WB:1:1000-1:5000 IHC:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
