WB
Western blot analysis of PYGL using anti-PYGL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates. \After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PYGL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for PYGL at approximately 97 kDa. The expected band size for PYGL is at 97 kDa.IHC
IHC analysis of PYGL using anti-PYGL antibody. PYGL was detected in a paraffin-embedded section of human pancrease cancer tissue. The tissue section was incubated with rabbit anti-PYGL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of A431 cells using anti-PYGL antibody. Overlay histogram showing A431 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYGL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-PYGL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PYGL recombinant protein (Position: K313-K804). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCIHCWB |
| WB:1:500-2000 FC:1:50-200 IHC:1:50-400 ELISA:1:100-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYGL |
|---|---|
| Gene Synonyms | GSD6 |
| Gene Full Name | glycogen phosphorylase L |
| Gene Summary | This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011] |
| Alternative Names | GSD6 PYGL |
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of PYGL using anti-PYGL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates. \After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PYGL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for PYGL at approximately 97 kDa. The expected band size for PYGL is at 97 kDa.
IHC
IHC analysis of PYGL using anti-PYGL antibody. PYGL was detected in a paraffin-embedded section of human pancrease cancer tissue. The tissue section was incubated with rabbit anti-PYGL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of A431 cells using anti-PYGL antibody. Overlay histogram showing A431 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYGL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 FC:1:50-200 IHC:1:50-400 ELISA:1:100-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
