WB
Various lysates were subjected to SDS PAGE followed by western blot with PYGL antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using PYGL antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PYGL antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), 594-phalloidin (red).IP
IP result of anti-PYGL (IP: 4ug; Detection: 1:1000) with mouse liver tissue lysate 4000ug.| Product Name | PYGL Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PYGL protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:3000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYGL |
|---|---|
| Gene Synonyms | GSD6 |
| Gene Full Name | glycogen phosphorylase L |
| Gene Summary | This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011] |
| Alternative Names | Glycogen phosphorylase liver form EC:2.4.1.1 |
| Molecular Weight(MW) | 98kDa |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PYGL antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using PYGL antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PYGL antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), 594-phalloidin (red).
IP
IP result of anti-PYGL (IP: 4ug; Detection: 1:1000) with mouse liver tissue lysate 4000ug.| Application Notes | WB:1:500-1:3000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
