WB
Western blot analysis of lysates from Rat testis, using DDX41 Rabbit pAb at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using DDX41 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using DDX41 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of U2OS cells using DDX41 Rabbit pAb. Secondary antibody:Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.IP
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg DDX41 antibody. Western blot was performed from the immunoprecipitate using DDX41 antibody at a dilution of 1:1000.| Product Name | DDX41 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:100 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX41 |
|---|---|
| Gene Synonyms | ABS MPLPF |
| Gene Full Name | DEAD-box helicase 41 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response. [provided by RefSeq, Jan 2017] |
| Alternative Names | ABS MPLPF DDX41 |
| Molecular Weight(MW) | 70kDa(Observed MW: 82kDa) |
| Cellular Localization | Nucleus,Cytoplasm. |
WB
Western blot analysis of lysates from Rat testis, using DDX41 Rabbit pAb at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using DDX41 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using DDX41 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of U2OS cells using DDX41 Rabbit pAb. Secondary antibody:Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IP
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg DDX41 antibody. Western blot was performed from the immunoprecipitate using DDX41 antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:100 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
