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Rabbit polyclonal antibody to DDX41

Catalog Number:OM645753
OM645753
Discount Price
OM645753-100ul
100ul
OM645753-50ul
50ul
Product Profile
Product Name Rabbit polyclonal antibody to DDX41
Antibody Type Primary Antibodies
Immunogen E.coli-derived human DDX41 recombinant protein (Position: K102-F622).
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications IHCWB
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity HumanMouseRat
Concentration 0.5mg/ml
Purification Affinity purified
Target Information
Gene Symbol DDX41
Gene Synonyms ABS
MPLPF
Gene Full Name DEAD-box helicase 41
Gene Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response. [provided by RefSeq, Jan 2017]
Alternative Names Probable ATP-dependent RNA helicase DDX41
EC:3.6.4.13
DEAD box protein 41
ABS
Molecular Weight(MW) 70 kDa
Cellular Localization Cytoplasm,Nucleus,Spliceosome
Application

WB

Western blot analysis of DDX41 using anti-DDX41 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX41 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.

IHC

IHC analysis of DDX41 using anti-DDX41 antibody. DDX41 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-DDX41 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of DDX41 using anti-DDX41 antibody. DDX41 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-DDX41 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Storage Buffer 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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