WB
human heart tissue were subjected to SDS PAGE followed by western blot with CD36 Antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
human spleen tissue were subjected to SDS PAGE followed by western blot with CD36 Antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using CD36 Antibody at dilution of 1:200 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using CD36 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD36 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD36 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Product Name | CD36 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CD36 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:6000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 IF-P:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD36 |
|---|---|
| Gene Synonyms | FAT GP4 GP3B GPIV CHDS7 PASIV SCARB3 BDPLT10 |
| Gene Full Name | CD36 molecule (CD36 blood group) |
| Gene Summary | The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014] |
| Alternative Names | GPIV GPIIIB GP4 GP3B Glycoprotein IIIb |
| Molecular Weight(MW) | 53kDa(Observed MW:88kDa) |
| Cellular Localization | Cell membrane,Golgi apparatus,Apical cell membrane. |
WB
human heart tissue were subjected to SDS PAGE followed by western blot with CD36 Antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
human spleen tissue were subjected to SDS PAGE followed by western blot with CD36 Antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using CD36 Antibody at dilution of 1:200 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using CD36 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD36 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD36 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Application Notes | WB:1:1000-1:6000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
