WB
Various lysates were subjected to SDS PAGE followed by western blot with SREBF1 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using SREBF1 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). CL594-Phalloidin stains the F-actin (red). DAPI (blue).| Product Name | SREBF1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SREBF1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:16000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Gene Synonyms | HMD IFAP2 SREBP1 bHLHd1 |
| Gene Full Name | sterol regulatory element binding transcription factor 1 |
| Gene Summary | This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] |
| Alternative Names | SREBP 1 SREBF 1 Processed sterol regulatory element-binding protein 1 Class D basic helix-loop-helix protein 1 bHLHd1 |
| Molecular Weight(MW) | 125kDa |
| Cellular Localization | Endoplasmic reticulum membrane.Golgi apparatus membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SREBF1 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using SREBF1 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). CL594-Phalloidin stains the F-actin (red). DAPI (blue).| Application Notes | WB:1:2000-1:16000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
