WB
Various lysates were subjected to SDS PAGE followed by western blot with UBE3C antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using UBE3C antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | UBE3C Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human UBE3C protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.3mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE3C |
|---|---|
| Gene Synonyms | RAUL HECTH2 NDSMBA NEDSMBA |
| Gene Full Name | ubiquitin protein ligase E3C |
| Gene Summary | Enables ubiquitin protein ligase activity. Involved in protein K29-linked ubiquitination; protein K48-linked ubiquitination; and ubiquitin-dependent protein catabolic process. Predicted to be part of proteasome complex. [provided by Alliance of Genome Resources, Jun 2025] |
| Alternative Names | KIAA10 KIAA0010 Homologous to E6AP carboxyl terminus homologous protein 2 HECT-type ubiquitin transferase E3C HectH2 |
| Molecular Weight(MW) | 124kDa(Observed MW:70kDa) |
| Cellular Localization | proteasome complex. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with UBE3C antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using UBE3C antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
