WB
Western blot analysis of UBE3C using anti-UBE3C antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBE3C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of UBE3C using anti-UBE3C antibody. UBE3C was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-UBE3C Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of UBE3C using anti-UBE3C antibody. UBE3C was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-UBE3C Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to UBE3C |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human UBE3C recombinant protein (Position: G18-Y982). Human UBE3C shares 92.7% amino acid (aa) sequence identity with mouse UBE3C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-8000 IHC:1:50-400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE3C |
|---|---|
| Gene Synonyms | RAUL HECTH2 NDSMBA NEDSMBA |
| Gene Full Name | ubiquitin protein ligase E3C |
| Gene Summary | Enables ubiquitin protein ligase activity. Involved in protein K29-linked ubiquitination; protein K48-linked ubiquitination; and ubiquitin-dependent protein catabolic process. Predicted to be part of proteasome complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | KIAA10 KIAA0010 Homologous to E6AP carboxyl terminus homologous protein 2 HECT-type ubiquitin transferase E3C HectH2 |
| Molecular Weight(MW) | 124 kDa(Observed band size:70 kDa) |
| Cellular Localization | Proteasome. |
WB
Western blot analysis of UBE3C using anti-UBE3C antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBE3C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of UBE3C using anti-UBE3C antibody. UBE3C was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-UBE3C Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of UBE3C using anti-UBE3C antibody. UBE3C was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-UBE3C Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:1000-8000 IHC:1:50-400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
