DB
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody at the same dose.| Product Name | N4-acetylcytidine Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Compound |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | DB |
| DB:1:1000-1:4000 |
|
| Predicted Reactivity | chemical compound |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Target Description | N4-Acetylcytidine, CasNo. 3768-18-1, is a modified nucleoside and endogenous urinary nucleoside product of the degradation of tRNA, 18s rRNA and mRNA. N4-Acetylcytidine is a biological marker for various cancers with elevated concentrations present in urine. N4-Acetylcytidine is also a partially protected cytidine and therefore can be used as a synthetic building block to prepare further derivatized nucleosides such as 2',3'-dideoxycytidine. NAT10 catalyzes the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. |
|---|---|
| Alternative Names | C11H15N3O6 Ac-rC Acetylcytidine ACETYL CYTOSINE Ac4C |
DB
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody at the same dose.| Application Notes | DB:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
