WB
Various lysates were subjected to SDS PAGE followed by western blot with Septin 4 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Septin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Septin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 4 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), acetylated Tubulin(Lys40) antibody (red).IP
IP result of anti-Septin 4 (IP:4ug; Detection:1:300) with A375 cells lysate 2.2mg.| Product Name | Septin 4 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SEPT4 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:1000-1:8000 IHC:1:50-1:500 IC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEPTIN4 |
|---|---|
| Gene Synonyms | H5 ARTS MART SEP4 CE5B3 SEPT4 PNUTL2 SPGF99 hucep-7 BRADEION C17orf47 Septin-4 hCDCREL-2 |
| Gene Full Name | septin 4 |
| Gene Summary | This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010] |
| Alternative Names | SEPT4 hucep7 hCDCREL-2 hCDCREL2 Cell division control-related protein 2 |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Cell projection,Cilium,Cytoplasm,Cytoplasmic vesicle,Flagellum,Mitochondrion,Nucleus,Synapse. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Septin 4 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Septin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Septin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 4 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), acetylated Tubulin(Lys40) antibody (red).
IP
IP result of anti-Septin 4 (IP:4ug; Detection:1:300) with A375 cells lysate 2.2mg.| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:500 IC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
