WB
GAL4 tag fusion protein (20 ng). GAL4 antibody was tested at concentrations of 1.0 µ g/ml (A), 0.1 µ g/ml (B), and 0.01 µ g/ml (C).WB
Western Blot Analysis of Yeast Recombinant GAL4 Fusion Protein.| Product Name | Mouse Monoclonal antibody to GAL4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 94-147 of GAL4. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB:1:100-1:1000 IP:1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
|
| Species Reactivity | Saccharomyces cerevisiaeYeast |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Target | The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein frag ment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (an amino acid sequence) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique “two-hybrid” system has been developed using GAL4 fusions in yeast to identify specific protein protein interactions. |
|---|---|
| Molecular Weight(MW) | 99kDa |
WB
GAL4 tag fusion protein (20 ng). GAL4 antibody was tested at concentrations of 1.0 µ g/ml (A), 0.1 µ g/ml (B), and 0.01 µ g/ml (C).
WB
Western Blot Analysis of Yeast Recombinant GAL4 Fusion Protein.| Application Notes | WB:1:100-1:1000 IP:1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
