WB
Western blot analysis of KCNQ2 using anti-KCNQ2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Sh-sy5y whole cell lysates, Lane 2: HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KCNQ2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of KCNQ2 using anti-KCNQ2 antibody. KCNQ2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCNQ2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of KCNQ2 using anti-KCNQ2 antibody. KCNQ2 was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-KCNQ2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with anti-KCNQ2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to KCNQ2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human KCNQ2 recombinant protein (Position: M371-D408). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNQ2 |
|---|---|
| Gene Synonyms | EBN BFNC DEE7 EBN1 ENB1 HNSPC KV7.2 KCNA11 |
| Gene Full Name | potassium voltage-gated channel subfamily Q member 2 |
| Gene Summary | The M channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability. The M channel is formed by the association of the protein encoded by this gene and a related protein encoded by the KCNQ3 gene, both integral membrane proteins. M channel currents are inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | BFNC EBN EBN1 ENB1 HNSPC KCNA11 KCNQ2 KQT like 2 KV7.2 KVEBN1 |
| Molecular Weight(MW) | 96kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of KCNQ2 using anti-KCNQ2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Sh-sy5y whole cell lysates, Lane 2: HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KCNQ2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of KCNQ2 using anti-KCNQ2 antibody. KCNQ2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-KCNQ2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of KCNQ2 using anti-KCNQ2 antibody. KCNQ2 was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-KCNQ2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with anti-KCNQ2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
