WB
Various lysates were subjected to SDS PAGE followed by western blot with KCNQ2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KCNQ2 antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) .| Product Name | KCNQ2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human KCNQ2 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:300-1:1200 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNQ2 |
|---|---|
| Gene Synonyms | EBN BFNC DEE7 EBN1 ENB1 HNSPC KV7.2 KCNA11 |
| Gene Full Name | potassium voltage-gated channel subfamily Q member 2 |
| Gene Summary | The M channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability. The M channel is formed by the association of the protein encoded by this gene and a related protein encoded by the KCNQ3 gene, both integral membrane proteins. M channel currents are inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1D4A1 BFNC EBN EBN1 ENB1 |
| Molecular Weight(MW) | 96kDa(Observed MW: 90kDa) |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with KCNQ2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KCNQ2 antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) .| Application Notes | WB:1:1000-1:4000 IHC:1:300-1:1200 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
