WB
The inhibitory effect of IFITM1 and mutants of IFITM1 on DENV replication. pcDNA3.1, pcDNA3.1-Flag-IFITM1 and mutants of pcDNA3.1-Flag-IFITM1 were transfected in Vero cells, respectively. After 24 h, cells were infected with DENV (MOI = 0.1). After 48 h, cells were collected, and the expression level of DENV E protein was analyzed by Western blotting .ICC/IF
HEK293T cells were mock-infected or infected with DENV 2 or DENV 4 and at 0, 20, 30 min and 1 hr p.i., cells were incubated with pan specific anti-dengue E protein antibody followed by an appropriate secondary antibody (green), phalloidin-TRITC (red) and DAPI (blue). Cells were observed under an Olympus Fluo View 1000 confocal microscope. Only the actin channel is shown. Full merged images with signal from all three channels are shown in Fig E in S1 File .| Product Name | Dengue Virus Type 1-4 Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Mixture of dengue 1, 2, 3 and 4. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:50-1:500 ICC/IF:1:10-1:50 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Virus |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Target | Dengue fever and dengue hemorrhagic fever are acute febrile diseases, found in the tropics and Africa. Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. Dengue is transmitted to humans by the Aedes aegypti (rarely Aedes albopictus) mosquito, which feeds during the day. |
|---|
WB
The inhibitory effect of IFITM1 and mutants of IFITM1 on DENV replication. pcDNA3.1, pcDNA3.1-Flag-IFITM1 and mutants of pcDNA3.1-Flag-IFITM1 were transfected in Vero cells, respectively. After 24 h, cells were infected with DENV (MOI = 0.1). After 48 h, cells were collected, and the expression level of DENV E protein was analyzed by Western blotting .
ICC/IF
HEK293T cells were mock-infected or infected with DENV 2 or DENV 4 and at 0, 20, 30 min and 1 hr p.i., cells were incubated with pan specific anti-dengue E protein antibody followed by an appropriate secondary antibody (green), phalloidin-TRITC (red) and DAPI (blue). Cells were observed under an Olympus Fluo View 1000 confocal microscope. Only the actin channel is shown. Full merged images with signal from all three channels are shown in Fig E in S1 File .| Application Notes | WB:1:50-1:500 ICC/IF:1:10-1:50 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 100 µg IgG2a in 1.0 ml of PBS with < 0.1% sodium azideand 0.1% gelatin. |
