WB
Western blot: 2.0 µg of GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, and Glucose-BSA stained with anti-O-GalNAc + O-GlcNAc antibody at 1:1000 for 2 hours at RT.ICC/IF
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with anti-O-GalNAc + O-GlcNAc antibody(green) at 1:50 dilution for 30 - 60 min at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.IF-P
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse colon tissue stained with anti-O-GalNAc + O-GlcNAc antibody (green) at 1:25 dilution for 1 hour at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.| Product Name | Mouse Monoclonal antibody to O-GalNAc + O-GlcNAc |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | ICC/IFIF-PWB |
| WB:1:1000 ICC/IF:1:50 IF-P:1:25 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | O-GalNAc + O-GlcNAc |
|---|
WB
Western blot: 2.0 µg of GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, and Glucose-BSA stained with anti-O-GalNAc + O-GlcNAc antibody at 1:1000 for 2 hours at RT.
ICC/IF
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with anti-O-GalNAc + O-GlcNAc antibody(green) at 1:50 dilution for 30 - 60 min at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.
IF-P
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse colon tissue stained with anti-O-GalNAc + O-GlcNAc antibody (green) at 1:25 dilution for 1 hour at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.| Application Notes | WB:1:1000 ICC/IF:1:50 IF-P:1:25 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
