WB
Western blot analysis of extracts from various samples, using PERK Mouse mAb. Lane 1: Mouse brain, blocked with antigen-specific peptides, Lane 2: Mouse brain, Lane 3: Rat brain, Lane 4: 293 cells.IHC
PERK Mouse monoclonal Antibody at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.ICC/IF
PERK Mouse monoclonal Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | PERK Mouse monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PERK. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK3 |
|---|---|
| Gene Synonyms | PEK WRS PERK |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 3 |
| Gene Summary | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] |
| Alternative Names | DKFZp781H1925 E2AK3_HUMAN EC 2.7.11.1 Eif2ak3 Eukaryotic translation initiation factor 2 alpha kinase 3 Eukaryotic translation initiation factor 2-alpha kinase 3 Heme regulated EIF2 alpha kinase HRI HsPEK Pancreatic eIF2 alpha kinase Pancreatic eIF2-alpha kinase PEK PRKR like endoplasmic reticulum kinase PRKR-like endoplasmic reticulum kinase WRS |
| Molecular Weight(MW) | 125kDa |
| Cellular Localization | Endoplasmic reticulum membrane>Single-pass type I membrane protein. |
WB
Western blot analysis of extracts from various samples, using PERK Mouse mAb. Lane 1: Mouse brain, blocked with antigen-specific peptides, Lane 2: Mouse brain, Lane 3: Rat brain, Lane 4: 293 cells.
IHC
PERK Mouse monoclonal Antibody at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.
ICC/IF
PERK Mouse monoclonal Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
