WB
Western blot analysis of CHMP5 expression in K-562 (A) and SK-N-SH (B) whole cell lysate.WB
Western blot analysis of CHMP5 expression in whole cell lysate of K-562 (A), A549 (B), and SW480 (C).ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol showed cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to CHMP5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 130-219 mapping at the C-terminus of CHMP5 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 IP:1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHMP5 |
|---|---|
| Gene Synonyms | Spike Vps60 CGI-34 PNAS-2 C9orf83 HSPC177 SNF7DC2 |
| Gene Full Name | charged multivesicular body protein 5 |
| Gene Summary | CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008] |
| Molecular Weight(MW) | 32kDa |
WB
Western blot analysis of CHMP5 expression in K-562 (A) and SK-N-SH (B) whole cell lysate.
WB
Western blot analysis of CHMP5 expression in whole cell lysate of K-562 (A), A549 (B), and SW480 (C).
ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol showed cytoplasmic localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 IP:1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
