WB
Western blot analysis of CTHRC1 using anti-CTHRC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat brain tissue lysates, Lane 2: Rat lung tissue lysates, Lane 3: Rat thymus tissue lysates, Lane 4: Rat PC-12 whole cell lysates, Lane 5: Mouse thymus tissue lysates, Lane 6: Mouse RAW264.7 whole cell lysates, Lane 7: Mouse SP2/0 whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTHRC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CTHRC1 using anti-CTHRC1 antibody. CTHRC1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTHRC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.ICC/IF
IF analysis of CTHRC1 using anti-CTHRC1 antibody. CTHRC1 was detected in an immunocytochemical section of HEPA1-6 cells. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI.FC
Flow Cytometry analysis of HepG2 cells using anti-CTHRC1 antibody. Overlay histogram showing HepG2 cells stained with anti-CTHRC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTHRC1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to CTHRC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human CTHRC1 recombinant protein (Position: S31-K243). Human CTHRC1 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CTHRC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTHRC1 |
|---|---|
| Gene Full Name | collagen triple helix repeat containing 1 |
| Gene Summary | This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012] |
| Alternative Names | CTHRC1 Protein NMTC1 |
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
WB
Western blot analysis of CTHRC1 using anti-CTHRC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat brain tissue lysates, Lane 2: Rat lung tissue lysates, Lane 3: Rat thymus tissue lysates, Lane 4: Rat PC-12 whole cell lysates, Lane 5: Mouse thymus tissue lysates, Lane 6: Mouse RAW264.7 whole cell lysates, Lane 7: Mouse SP2/0 whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTHRC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CTHRC1 using anti-CTHRC1 antibody. CTHRC1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTHRC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
ICC/IF
IF analysis of CTHRC1 using anti-CTHRC1 antibody. CTHRC1 was detected in an immunocytochemical section of HEPA1-6 cells. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI.
FC
Flow Cytometry analysis of HepG2 cells using anti-CTHRC1 antibody. Overlay histogram showing HepG2 cells stained with anti-CTHRC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTHRC1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
