WB
Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with SERCA2/ATP2A2 antibody at dilution of 1:15000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using SERCA2/ATP2A2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2/ATP2A2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2/ATP2A2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | SERCA2/ATP2A2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SERCA2,ATP2A2 protein（AA:314-580） |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:5000-1:20000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IF-P:1:50-1:500 IF-F:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.75mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2A2 |
|---|---|
| Gene Synonyms | DD DAR ATP2B SERCA2 RHABDO2 |
| Gene Full Name | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 |
| Gene Summary | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019] |
| Alternative Names | ATP2A2 SERCA2 SERCA2 ATP2A2 SERCA2b ATP2A 2 |
| Molecular Weight(MW) | 110-115 kDa |
| Cellular Localization | Endoplasmic reticulum membrane,Sarcoplasmic reticulum membrane. |
WB
Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with SERCA2/ATP2A2 antibody at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using SERCA2/ATP2A2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2/ATP2A2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2/ATP2A2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:5000-1:20000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IF-P:1:50-1:500 IF-F:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
