WB
Western blot analysis of AMID on different lysates with Rabbit anti-AMID antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane), Lane 2: MCF7 cell lysate (no heat) (15 µg/Lane), Lane 3: HeLa cell lysate (no heat) (20 µg/Lane), Lane 4: NCI-H1299 cell lysate (no heat) (20 µg/Lane), Lane 5: LO2 cell lysate (15 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-AMID antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-AMID antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling AMID with Rabbit anti-AMID antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMID antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Species: Rat, Site: liver, Sample: Paraffin-embedded section, Antibody concentration: 1/200.FC
Flow cytometric analysis of PC-12 cells labeling AMID. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
AMID was immunoprecipitated from 0.2 mg HepG2 cell lysate with Rabbit anti-AMID antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-AMID antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input), Lane 2: Rabbit anti-AMID antibody IP in HepG2 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-AMID antibody in HepG2 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 25 seconds.| Product Name | AMID Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human AMID aa 1-373. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCIPWB |
| WB:1:5000 IHC:1:200 ICC/IF:1:100 IF-P:1:200 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIFM2 |
|---|---|
| Gene Synonyms | AMID FSP1 PRG3 |
| Gene Full Name | AIF family member 2 |
| Gene Summary | This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010] |
| Alternative Names | 5430437E11Rik antibody aifm2 antibody AIFM2_HUMAN antibody AMID antibody Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody Apoptosis inducing factor mitochondrion associated 2 antibody Apoptosis-inducing factor 2 antibody Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody 5430437E11Rik antibody aifm2 antibody AIFM2_HUMAN antibody AMID antibody Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody Apoptosis inducing factor mitochondrion associated 2 antibody Apoptosis-inducing factor 2 antibody Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody Cys51Stop antibody Ferroptosis suppressor protein 1 antibody FSP1 antibody HGNC11998 antibody p53 responsive gene 3 antibody p53 tumor suppressor antibody p53-responsive gene 3 protein antibody PRG3 antibody TRP53 antibody Tumor protein p53 antibody |
| Molecular Weight(MW) | 41kDa(Observed band size: 45kDa) |
| Cellular Localization | Lipid droplet, Cell membrane, Cytoplasm, Mitochondrion membrane, Nucleus. |
WB
Western blot analysis of AMID on different lysates with Rabbit anti-AMID antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane), Lane 2: MCF7 cell lysate (no heat) (15 µg/Lane), Lane 3: HeLa cell lysate (no heat) (20 µg/Lane), Lane 4: NCI-H1299 cell lysate (no heat) (20 µg/Lane), Lane 5: LO2 cell lysate (15 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-AMID antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-AMID antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling AMID with Rabbit anti-AMID antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMID antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Species: Rat, Site: liver, Sample: Paraffin-embedded section, Antibody concentration: 1/200.
FC
Flow cytometric analysis of PC-12 cells labeling AMID. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
AMID was immunoprecipitated from 0.2 mg HepG2 cell lysate with Rabbit anti-AMID antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-AMID antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input), Lane 2: Rabbit anti-AMID antibody IP in HepG2 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-AMID antibody in HepG2 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 25 seconds.| Application Notes | WB:1:5000 IHC:1:200 ICC/IF:1:100 IF-P:1:200 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
