WB
HepG2 cells were subjected to SDS PAGE followed by western blot with AIFM2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using AIFM2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).FC
1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.IP
IP result of anti-AIFM2/ FSP1 (IP:4ug; Detection:1:500) with K-562 cells lysate 1760 ug.| Product Name | AIFM2/ FSP1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | AIFM2 Fusion Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000-1:50000 IHC:1:250-1:1000 ICC:1:400-1:1600 FC:0.50 ug per 10^6 cells in a 100 µl suspension|IP |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | AIFM2 |
|---|---|
| Gene Synonyms | AMID FSP1 PRG3 |
| Gene Full Name | AIF family member 2 |
| Gene Summary | This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010] |
| Alternative Names | AIFM2 AMID FSP1 1A2B2 AIFM 2 |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Lipid droplet,Membrane,Mitochondrion,Nucleus. |
WB
HepG2 cells were subjected to SDS PAGE followed by western blot with AIFM2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using AIFM2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC
1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IP
IP result of anti-AIFM2/ FSP1 (IP:4ug; Detection:1:500) with K-562 cells lysate 1760 ug.| Application Notes | WB:1:5000-1:50000 IHC:1:250-1:1000 ICC:1:400-1:1600 FC:0.50 ug per 10^6 cells in a 100 µl suspension|IP |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
