WB
Western blot analysis of ACVR2B on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 1 hour at room temperature.| Product Name | ACVR2B Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human ACVR2B aa 331-380 / 512. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ACVR2B |
|---|---|
| Gene Synonyms | HTX4 ACTRIIB ActR-IIB |
| Gene Full Name | activin A receptor type 2B |
| Gene Summary | Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq, Jul 2008] |
| Alternative Names | Activin A receptor type IIB antibody Activin receptor type 2B antibody Activin receptor type IIB antibody Activin receptor type-2B antibody ActR IIB antibody ACTR-IIB antibody ActRIIB antibody ACVR 2B antibody ACVR2B antibody AVR2B_HUMAN antibody Activin A receptor type IIB antibody Activin receptor type 2B antibody Activin receptor type IIB antibody Activin receptor type-2B antibody ActR IIB antibody ACTR-IIB antibody ActRIIB antibody ACVR 2B antibody ACVR2B antibody AVR2B_HUMAN antibody HTX4 antibody MGC116908 antibody |
| Molecular Weight(MW) | 58kDa(Observed band size: 50 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of ACVR2B on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA. Preservative: 0.05% Sodium Azide. |
