WB
Western blot analysis of various lysates using Natriuretic peptides A Rabbit mAb at 1:50000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffin embedded Human heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Human heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Mouse heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Rat heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Product Name | Natriuretic peptides A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:10000-1:50000 IHC:1:2000-1:8000 IF-P:1:200-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPPA |
|---|---|
| Gene Synonyms | ANF ANP CDD CDP PND ATFB6 ATRST2 CDD-ANF |
| Gene Full Name | natriuretic peptide A |
| Gene Summary | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015] |
| Alternative Names | ANF ANP CDD CDP PND ATFB6 ATRST2 CDD-ANF |
| Molecular Weight(MW) | 16kDa |
| Cellular Localization | cell projection, cytoplasm, extracellular region, extracellular space, nucleus, perikaryon, Secreted. |
WB
Western blot analysis of various lysates using Natriuretic peptides A Rabbit mAb at 1:50000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffin embedded Human heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat heart tissue using Natriuretic peptides A Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Human heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Mouse heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Rat heart tissue using Natriuretic peptides A Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:10000-1:50000 IHC:1:2000-1:8000 IF-P:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
