WB
Western blot analysis of lysates from Mouse heart, using NPPA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using NPPA RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using NPPA Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Product Name | NPPA Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:5000 IHC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPPA |
|---|---|
| Gene Synonyms | ANF ANP CDD CDP PND ATFB6 ATRST2 CDD-ANF |
| Gene Full Name | natriuretic peptide A |
| Gene Summary | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015] |
| Molecular Weight(MW) | 16kDa(Observed band size:16kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of lysates from Mouse heart, using NPPA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using NPPA RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using NPPA Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Application Notes | WB:1:500-1:5000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
