WB
Western blot - Anti-Sulfotyrosine antibody at 1/1000 dilution, Lane 1:Purified bovine fibrinogen treated with 1.5 mg/ml abalone sulfatase at 37°C overnight. Lane 2:Purified bovine fibrinogen.Secondary All lanes:Goat anti-mouse secondary antibody conjugated to HRP at 1/2000 dilution.| Product Name | Anti-Sulfotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sulfonyltyrosine is a common post-translational modification of secreted proteins, plasma membrane proteins, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferases in the trans Golgi network. Tyrosine sulfation occurs in all multicellular organisms, and up to 1% of tyrosine in the total protein content of cells can be sulfated. Sulfonyltyrosine mediates many receptor ligand binding interactions, including the interaction between P-selectin glycoprotein ligand 1 and P-selectin, the binding of factor VIII to hemophilia factor, and the interaction between thrombin and hirudin. Sulfonyltyrosine is also involved in the entry of several parasites and viruses. For example, the entry of the virus into host cells requires tyrosine sulfation of the HIV-1 co receptor CCR5, while tyrosine sulfation of the Duffy antigen/chemokine receptor is crucial for the invasion of red blood cells by the malaria parasite Plasmodium vivax. |
| Immunogen | Epitope: Sulfated tyrosine. This antibody was developed using the phage display library described by Kehoe et al. in 2006. Select antigens KAKISDP-DY (SO3) MTGYMDAC and KDKKYATEY (SO3) - EYLDYDFC |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB:1:1000 IP:1:10-1:100 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein G |
WB
Western blot - Anti-Sulfotyrosine antibody at 1/1000 dilution, Lane 1:Purified bovine fibrinogen treated with 1.5 mg/ml abalone sulfatase at 37°C overnight. Lane 2:Purified bovine fibrinogen.Secondary All lanes:Goat anti-mouse secondary antibody conjugated to HRP at 1/2000 dilution.| Application Notes | WB:1:1000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | pH: 7.5, Preservative: 0.05% Sodium azide, Constituents: 1.35% Glycine, 1.21% Tris, 0.88% Sodium chloride. |
