WB
Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.ICC/IF
Immunofluorescence analysis of L929 cells using SH3PXD2A Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | SH3PXD2A Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SH3PXD2A |
|---|---|
| Gene Synonyms | FISH TKS5 SH3MD1 |
| Gene Full Name | SH3 and PX domains 2A |
| Gene Summary | Predicted to enable superoxide-generating NADPH oxidase activator activity. Involved in osteoclast fusion and superoxide metabolic process. Located in cytosol and podosome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | FISH TKS5 SH3MD1 SH3PXD2A |
| Molecular Weight(MW) | 125kDa(Observed MW 100kDa) |
| Cellular Localization | Cell projection, Cytoplasm, podosome. |
WB
Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
ICC/IF
Immunofluorescence analysis of L929 cells using SH3PXD2A Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
