WB
Western blot analysis of APPL on different lysates with Rabbit anti-APPL antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: HEK-293 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: PC-12 cell lysate, Lane 6: Mouse brain tissue lysate, Lane 7: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
APPL was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Rabbit anti-APPL antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-APPL antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input), Lane 2: Rabbit anti-APPL antibody IP in HEK-293 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-APPL antibody in HEK-293 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Product Name | APPL Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human APPL aa 400-500. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000 IHC:1:500 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | APPL1 |
|---|---|
| Gene Synonyms | APPL MODY14 DIP13alpha |
| Gene Full Name | adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 |
| Gene Summary | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] |
| Alternative Names | Adapter protein containing PH domain antibody Adaptor Protein Containing pH Domain antibody Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody AKT2 INTERACTOR antibody APPL 1 antibody Appl1 antibody DCC interacting protein 13 alpha antibody DCC-interacting protein 13-alpha antibody Adapter protein containing PH domain antibody Adaptor Protein Containing pH Domain antibody Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody AKT2 INTERACTOR antibody APPL 1 antibody Appl1 antibody DCC interacting protein 13 alpha antibody DCC-interacting protein 13-alpha antibody DIP 13 alpha antibody DIP13 alpha antibody Dip13-alpha antibody DIP13alpha antibody DP13A_HUMAN antibody KIAA1428 antibody PTB domain and leucine zipper motif 1 antibody PTB Domain and Leucine Zipper Motif antibody Signaling adaptor protein DIP13alpha antibody |
| Molecular Weight(MW) | 80kDa |
| Cellular Localization | Early endosome membrane, Nucleus, Cytoplasm, Endosome, Cell projection, ruffle, Cytoplasmic vesicle, phagosome. |
WB
Western blot analysis of APPL on different lysates with Rabbit anti-APPL antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: HEK-293 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: PC-12 cell lysate, Lane 6: Mouse brain tissue lysate, Lane 7: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-APPL antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
APPL was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Rabbit anti-APPL antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-APPL antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input), Lane 2: Rabbit anti-APPL antibody IP in HEK-293 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-APPL antibody in HEK-293 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Application Notes | WB:1:1000 IHC:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
