WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Mouse mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using TriMethyl-Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse kidney tissue using TriMethyl-Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney tissue using TriMethyl Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of C6 cells using TriMethyl Histone H3-K27 Mouse mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti Mouse IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K27 Mouse mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit for Illumina from 10⁵ K562 cells with 1 μg TriMethyl Histone H3-K27 Mouse mAb antibody, along with a Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3 K27 in representative gene loci, as shown in figure.| Product Name | TriMethyl-Histone H3-K27 Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Modification | TriMethyl K27 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ChIPDBICC/IFIHCWBCUT&Tag-seq |
| WB:1:1000-1:5000 IHC:1:50-1:200 ICC/IF:1:50-1:200 DB:1:500-1:2000 ChIP:5μg antibody for 5μg-10μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C1 |
|---|---|
| Gene Synonyms | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3t H3.4 H3/g H3FT H3C16 HIST3H3 TriMethyl-Histone H3-K27 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Chromosome, nucleus. |
WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Mouse mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using TriMethyl-Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse kidney tissue using TriMethyl-Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney tissue using TriMethyl Histone H3-K27 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of C6 cells using TriMethyl Histone H3-K27 Mouse mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti Mouse IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K27 Mouse mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit for Illumina from 10⁵ K562 cells with 1 μg TriMethyl Histone H3-K27 Mouse mAb antibody, along with a Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3 K27 in representative gene loci, as shown in figure.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:200 ICC/IF:1:50-1:200 DB:1:500-1:2000 ChIP:5μg antibody for 5μg-10μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
