WB
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: K-562 cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate, Lane 2: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | IRAK4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human IRAK4 aa 1-100 (N terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
|
| Species Reactivity | HumanMonkeyMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IRAK4 |
|---|---|
| Gene Synonyms | IPD1 IMD67 REN64 IRAK-4 NY-REN-64 |
| Gene Full Name | interleukin 1 receptor associated kinase 4 |
| Gene Summary | This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | IL-1 receptor-associated kinase 4 antibody Interleukin 1 receptor associated kinase 4 mutant form 1 antibody Interleukin-1 receptor-associated kinase 4 antibody Interleukin1 receptor associated kinase 4 antibody IPD1 antibody IRAK 4 antibody IRAK-4 antibody IRAK4 antibody IRAK4 mutated form 1 antibody IRAK4_HUMAN antibody IL-1 receptor-associated kinase 4 antibody Interleukin 1 receptor associated kinase 4 mutant form 1 antibody Interleukin-1 receptor-associated kinase 4 antibody Interleukin1 receptor associated kinase 4 antibody IPD1 antibody IRAK 4 antibody IRAK-4 antibody IRAK4 antibody IRAK4 mutated form 1 antibody IRAK4_HUMAN antibody LOC 51135 antibody NY REN 64 antibody NY REN 64 antigen antibody NY-REN-64 antibody REN64 antibody Renal carcinoma antigen NY-REN-64 antibody |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: K-562 cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate, Lane 2: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-IRAK4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
