WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Endoplasmin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.IHC
Human prostate was stained with anti-Endoplasmin rabbit antibody.IHC
Human colon was stained with anti-Endoplasmin rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Endoplasmin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Endoplasmin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSP90B1 |
|---|---|
| Gene Synonyms | ECGP GP96 TRA1 GRP94 HEL35 HEL-S-125m |
| Gene Full Name | heat shock protein 90 beta family member 1 |
| Gene Summary | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| Alternative Names | HSP90B1 GRP94 TRA1 Endoplasmin 94 kDa glucose-regulated protein GRP-94 Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog |
| Molecular Weight(MW) | 93kDa(Observed band size: 100kDa) |
| Cellular Localization | Endoplasmic reticulum. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Endoplasmin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
IHC
Human prostate was stained with anti-Endoplasmin rabbit antibody.
IHC
Human colon was stained with anti-Endoplasmin rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Endoplasmin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
