WB
Western blot analysis of GRP94 on different lysates with Mouse anti-GRP94 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Mouse placenta tissue lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Lane 8: Rat placenta tissue lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (20 µg/Lane) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GRP94 antibody. Counter stained with hematoxylin.ICC/IF
ICC staining GRP94 (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | GRP94 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human GRP94 aa 201-400 / 803. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:10000 IHC:1:50-1:200 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSP90B1 |
|---|---|
| Gene Synonyms | ECGP GP96 TRA1 GRP94 HEL35 HEL-S-125m |
| Gene Full Name | heat shock protein 90 beta family member 1 |
| Gene Summary | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| Alternative Names | 94 kDa glucose regulated protein antibody 94 kDa glucose-regulated protein antibody ECGP antibody Endoplasmin antibody Endothelial cell (HBMEC) glycoprotein antibody ENPL_HUMAN antibody Glucose regulated protein 94kDa antibody gp96 antibody gp96 homolog antibody GRP 94 antibody 94 kDa glucose regulated protein antibody 94 kDa glucose-regulated protein antibody ECGP antibody Endoplasmin antibody Endothelial cell (HBMEC) glycoprotein antibody ENPL_HUMAN antibody Glucose regulated protein 94kDa antibody gp96 antibody gp96 homolog antibody GRP 94 antibody GRP-94 antibody Heat shock protein 90 kDa beta member 1 antibody heat shock protein 90kDa beta (Grp94) member 1 antibody Heat shock protein 90 kDa beta 1 antibody HSP90B1 antibody Stress inducible tumor rejection antigen GP96 antibody TRA1 antibody tumor rejection antigen (gp96) 1 antibody Tumor rejection antigen 1 antibody Tumor rejection antigen gp96 antibody Tumor rejection antigen-1 (gp96) antibody |
| Molecular Weight(MW) | 94kDa |
| Cellular Localization | Endoplasmic reticulum lumen, melanosome. |
WB
Western blot analysis of GRP94 on different lysates with Mouse anti-GRP94 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Mouse placenta tissue lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Lane 8: Rat placenta tissue lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (20 µg/Lane) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ICC/IF
ICC staining GRP94 (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Application Notes | WB:1:1000-1:10000 IHC:1:50-1:200 ICC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
