ELISA
Indirect ELISA and competitive ELISA results show that this antibody is specific to β-Nicotinamide Mononucleotide (NMN). Indirect ELISA was performed by coating BSA conjugated β-Nicotinamide Mononucleotide (NMN) at ~10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of β-Nicotinamide Mononucleotide (NMN) were mixed in primary antibody (fixed at 10 ng/mL).| Product Name | β-Nicotinamide Mononucleotide Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | PTG |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | β-NMN β NMN NMN |
|---|
ELISA
Indirect ELISA and competitive ELISA results show that this antibody is specific to β-Nicotinamide Mononucleotide (NMN). Indirect ELISA was performed by coating BSA conjugated β-Nicotinamide Mononucleotide (NMN) at ~10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of β-Nicotinamide Mononucleotide (NMN) were mixed in primary antibody (fixed at 10 ng/mL).| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
