WB
HeLa cell lysates were subjected to Tris-Acetate gel system followed by western blot with FAT1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using FAT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | FAT1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FAT1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 0.35mg/ml |
| Purification | Protein A |
| Gene Symbol | FAT1 |
|---|---|
| Gene Synonyms | FAT ME5 CDHF7 CDHR8 hFat1 |
| Gene Full Name | FAT atypical cadherin 1 |
| Gene Summary | This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008] |
| Alternative Names | CDHF7 FAT hFat1 ME5 |
| Molecular Weight(MW) | 506-600 kDa |
| Cellular Localization | Cell membrane,Nucleus. |
WB
HeLa cell lysates were subjected to Tris-Acetate gel system followed by western blot with FAT1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using FAT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
