WB
Near-infrared Western blot analysis of G3BP1 expression in whole-cell lysates of K-562 (A), A-431 (B), Ramos (C), MDA-MB-231 (D) and A549 (E).IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymph node tissues shows cytoplasmic staining in germinal center cells and non-germinal center cells.ICC/IF
Immunofluorescence staining of formalin-fixed SW480 cells shows cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to G3BP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 158-251 mapping within an internal region of G3BP1 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | G3BP1 |
|---|---|
| Gene Synonyms | G3BP HDH-VIII |
| Gene Full Name | G3BP stress granule assembly factor 1 |
| Gene Summary | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Near-infrared Western blot analysis of G3BP1 expression in whole-cell lysates of K-562 (A), A-431 (B), Ramos (C), MDA-MB-231 (D) and A549 (E).
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymph node tissues shows cytoplasmic staining in germinal center cells and non-germinal center cells.
ICC/IF
Immunofluorescence staining of formalin-fixed SW480 cells shows cytoplasmic localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
