WB
Western blot analysis of MAP3K1 on different lysates with Rabbit anti-MAP3K1 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: SH-SY5Y cell lysate (20 µg/Lane), Lane 5: THP-1 cell lysate (20 µg/Lane), Lane 6: HeLa cell lysate (20 µg/Lane), Lane 7: HL-60 cell lysate (20 µg/Lane), Lane 8: PC-12 cell lysate (20 µg/Lane), Lane 9: Mouse spleen tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | MAP3K1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human MAP3K1 aa 1413-1512. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP3K1 |
|---|---|
| Gene Synonyms | MEKK MEKK1 SRXY6 MEKK 1 MAPKKK1 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 1 |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012] |
| Alternative Names | M3K1_HUMAN antibody MAP3K1 antibody MAPK/ERK kinase kinase 1 antibody MAPKKK1 antibody MEK kinase 1 antibody MEKK 1 antibody Mekk antibody MEKK1 antibody Mitogen activated protein kinase kinase kinase 1 antibody Mitogen activated protein kinase kinase kinase 1 E3 ubiquitin protein ligase antibody M3K1_HUMAN antibody MAP3K1 antibody MAPK/ERK kinase kinase 1 antibody MAPKKK1 antibody MEK kinase 1 antibody MEKK 1 antibody Mekk antibody MEKK1 antibody Mitogen activated protein kinase kinase kinase 1 antibody Mitogen activated protein kinase kinase kinase 1 E3 ubiquitin protein ligase antibody Mitogen-activated protein kinase kinase kinase 1 antibody SRXY6 antibody |
| Molecular Weight(MW) | 165 kDa(Observed band size: 130 kDa) |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Western blot analysis of MAP3K1 on different lysates with Rabbit anti-MAP3K1 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: SH-SY5Y cell lysate (20 µg/Lane), Lane 5: THP-1 cell lysate (20 µg/Lane), Lane 6: HeLa cell lysate (20 µg/Lane), Lane 7: HL-60 cell lysate (20 µg/Lane), Lane 8: PC-12 cell lysate (20 µg/Lane), Lane 9: Mouse spleen tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
