WB
Western blot analysis of TGF beta Receptor II on different lysates with Rabbit anti-TGF beta Receptor II antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: Rat lung tissue lysate (40 µg/Lane), Exposure time: 1 minute 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | TGF beta Receptor II Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human TGF beta Receptor II aa 1-166 / 567. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TGFBR2 |
|---|---|
| Gene Synonyms | AAT3 FAA3 LDS2 MFS2 RIIC LDS1B LDS2B TAAD2 TBRII TBR-ii TGFR-2 tbetaR-II TGFbeta-RII |
| Gene Full Name | transforming growth factor beta receptor 2 |
| Gene Summary | The protein encoded by this gene is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of genes related to cell proliferation, cell cycle arrest, wound healing, immunosuppression, and tumorigenesis. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Aug 2017] |
| Alternative Names | AAT3 antibody FAA3 antibody LDS1B antibody LDS2 antibody LDS2B antibody MFS2 antibody RIIC antibody TAAD2 antibody TbetaR II antibody TbetaR-II antibody AAT3 antibody FAA3 antibody LDS1B antibody LDS2 antibody LDS2B antibody MFS2 antibody RIIC antibody TAAD2 antibody TbetaR II antibody TbetaR-II antibody TGF beta receptor type 2 antibody TGF beta receptor type II antibody TGF beta receptor type IIB antibody TGF beta type II receptor antibody TGF-beta receptor type II antibody TGF-beta receptor type-2 antibody TGF-beta type II receptor antibody TGF-beta-R2 antibody TGFB R2 antibody TGFbeta - RII antibody TGFbeta RII antibody Tgfbr2 antibody TGFR-2 antibody TGFR2_HUMAN antibody Transforming growth factor beta receptor II antibody Transforming growth factor beta receptor type II antibody Transforming growth factor beta receptor type IIC antibody Transforming growth factor beta receptor II (70/80kDa) antibody transforming growth factor beta receptor II alpha antibody transforming growth factor beta receptor II beta antibody transforming growth factor beta receptor II delta antibody transforming growth factor beta receptor II epsilon antibody transforming growth factor beta receptor II gamma antibody Transforming growth factor-beta receptor type II antibody |
| Molecular Weight(MW) | 65 kDa(Observed band size: 80 kDa) |
| Cellular Localization | Cell membrane, Membrane raft; Secreted. |
WB
Western blot analysis of TGF beta Receptor II on different lysates with Rabbit anti-TGF beta Receptor II antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: Rat lung tissue lysate (40 µg/Lane), Exposure time: 1 minute 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
