WB
Western blot analysis of various lysates using TGF beta Receptor II (TGFBR2) Rabbit pAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.WB
Western blot analysis of various lysates using TGF beta Receptor II (TGFBR2) Rabbit pAb at 1:900dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HeLa cellsusing TGF beta Receptor II (TGFBR2) Rabbit pAb at dilution of 1:200 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing TGF beta Receptor II (TGFBR2) RabbitpAb at dilution of 1:200 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.| Product Name | TGF beta Receptor II Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGFBR2 |
|---|---|
| Gene Synonyms | AAT3 FAA3 LDS2 MFS2 RIIC LDS1B LDS2B TAAD2 TBRII TBR-ii TGFR-2 tbetaR-II TGFbeta-RII |
| Gene Full Name | transforming growth factor beta receptor 2 |
| Gene Summary | The protein encoded by this gene is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of genes related to cell proliferation, cell cycle arrest, wound healing, immunosuppression, and tumorigenesis. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Aug 2017] |
| Alternative Names | AAT3 FAA3 LDS2 MFS2 RIIC LDS1B LDS2B TAAD2 TBRII TBR-ii TGFR-2 tbetaR-II TGFbeta-RII TGF beta Receptor II (TGFBR2) |
| Molecular Weight(MW) | 65kDa(Observed band size:70/85kDa) |
| Cellular Localization | Cell membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using TGF beta Receptor II (TGFBR2) Rabbit pAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
WB
Western blot analysis of various lysates using TGF beta Receptor II (TGFBR2) Rabbit pAb at 1:900dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing TGF beta Receptor II (TGFBR2) Rabbit pAb at dilution of 1:200 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing TGF beta Receptor II (TGFBR2) RabbitpAb at dilution of 1:200 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
