WB
Western blot analysis of non-muscle Myosin IIA on different lysates with Rabbit anti-non-muscle Myosin IIA antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HUVEC cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: HT-29 cell lysate (20 µg/Lane), Lane 5: NIH/3T3 cell lysate (20 µg/Lane), Lane 6: C2C12 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Lane 8: A549 cell lysate (20 µg/Lane), Lane 9: Mouse kidney tissue lysate (40 µg/Lane), Lane 10: Mouse lung tissue lysate (40 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Rat kidney tissue lysate (40 µg/Lane), Lane 13: Rat lung tissue lysate (40 µg/Lane), Lane 14: Rat liver tissue lysate (40 µg/Lane), Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling non-muscle Myosin IIA with Rabbit anti-non-muscle Myosin IIA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-non-muscle Myosin IIA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of NIH/3T3 cells labeling non-muscle Myosin IIA. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
non-muscle Myosin IIA was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-non-muscle Myosin IIA antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-non-muscle Myosin IIA antibody at 1/50,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-non-muscle Myosin IIA antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-non-muscle Myosin IIA antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Product Name | non-muscle Myosin IIA Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MYH9 aa 1,151-1,430. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYH9 |
|---|---|
| Gene Synonyms | MHA FTNS EPSTS BDPLT6 DFNA17 MATINS NMMHCA NMHC-II-A NMMHC-IIA |
| Gene Full Name | myosin heavy chain 9 |
| Gene Summary | This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011] |
| Alternative Names | BDPLT 6 antibody BDPLT6 antibody Cellular myosin heavy chain antibody Cellular myosin heavy chain type A antibody DFNA 17 antibody DFNA17 antibody EPSTS antibody FTNS antibody MGC104539 antibody MHA antibody BDPLT 6 antibody BDPLT6 antibody Cellular myosin heavy chain antibody Cellular myosin heavy chain type A antibody DFNA 17 antibody DFNA17 antibody EPSTS antibody FTNS antibody MGC104539 antibody MHA antibody MYH 2A antibody MYH 9 antibody MYH2A antibody MYH9 antibody MYH9_HUMAN antibody MYHas8 antibody MyHC 2A antibody MyHC IIa antibody MyHC2A antibody MyHCIIa antibody MYHSA 2 antibody MYHSA2 antibody Myosin 9 antibody Myosin heavy chain 9 antibody Myosin heavy chain 9 non muscle antibody Myosin heavy chain antibody Myosin heavy chain non muscle IIa antibody Myosin heavy chain nonmuscle IIa antibody Myosin heavy polypeptide 2 antibody Myosin heavy polypeptide 9 non muscle antibody Myosin-9 antibody Myosin9 antibody NMHC II A antibody NMMHC A antibody NMMHC II a antibody NMMHC II-a antibody NMMHC IIA antibody NMMHC-A antibody NMMHC-IIA antibody NMMHCA antibody Non muscle myosin heavy chain A antibody Non muscle myosin heavy chain antibody Non muscle myosin heavy chain II A antibody Non muscle myosin heavy polypeptide 9 antibody non-muscle IIa antibody Non-muscle myosin heavy chain A antibody Non-muscle myosin heavy chain IIa antibody Nonmuscle myosin heavy chain A antibody Nonmuscle myosin heavy chain II A antibody type A antibody |
| Molecular Weight(MW) | 227 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, cell cortex, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cell membrane. |
WB
Western blot analysis of non-muscle Myosin IIA on different lysates with Rabbit anti-non-muscle Myosin IIA antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HUVEC cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: HT-29 cell lysate (20 µg/Lane), Lane 5: NIH/3T3 cell lysate (20 µg/Lane), Lane 6: C2C12 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Lane 8: A549 cell lysate (20 µg/Lane), Lane 9: Mouse kidney tissue lysate (40 µg/Lane), Lane 10: Mouse lung tissue lysate (40 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Rat kidney tissue lysate (40 µg/Lane), Lane 13: Rat lung tissue lysate (40 µg/Lane), Lane 14: Rat liver tissue lysate (40 µg/Lane), Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling non-muscle Myosin IIA with Rabbit anti-non-muscle Myosin IIA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-non-muscle Myosin IIA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of NIH/3T3 cells labeling non-muscle Myosin IIA. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
non-muscle Myosin IIA was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-non-muscle Myosin IIA antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-non-muscle Myosin IIA antibody at 1/50,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-non-muscle Myosin IIA antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-non-muscle Myosin IIA antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
